کتاب دانلود کتب لاتین علوم حیات ژنتیک مولکولی
طراحی RNA - راهنمای CRISPR - روش ها و شیوه نامه ها
طراحی RNA - راهنمای CRISPR - روش ها و شیوه نامه ها

CRISPR Guide RNA Design: Methods and Protocols
نویسنده:
سال انتشار: 2021
تعداد صفحات: 287
زبان فایل: انگلیسی
فرمت فایل: pdf
حجم فایل: 10MB
رمز فایل: www.ketabdownload.com
قیمت: 210,000ريال

افزودن به سبد دانلود

Publisher: Humana

ISBN-10: 1071606867

ISBN-13: 978-1071606865

ASIN:

(Methods in Molecular Biology (2162)) 1st ed. 2021 Edition

by Tudor A. Fulga (Editor), David J. H. F. Knapp (Editor), Quentin R. V. Ferry (Editor)

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.