کتاب دانلود کتب لاتین علوم مهندسیسایر موارد
میکروسیالات برای آنالیز تک سلولی
میکروسیالات برای آنالیز تک سلولی

Microfluidics for Single-Cell Analysis
نویسنده: Jin, Ming Lin
سال انتشار: 2019
تعداد صفحات: 263
زبان فایل: English
فرمت فایل: pdf
حجم فایل: 13MB

دانلود رایگان

Publisher : Springer

ISBN-10 : 9813297301, 981329728X

ISBN-13 : 978-9813297302, 978-9813297289

ASIN : B07WZYXSQD

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.